Metabonomic Analysis of Water Extracts from Different Angelica Roots by 1H-Nuclear Magnetic Resonance Spectroscopy

Molecules

Pui Hei Chan¹  Wendy L. Zhang³  Chung-Ho Lau²  Chi Yuen Cheung¹  Hector C. Keun²  Karl W. K. Tsim³ 

[1]Department of Chemical and Biomolecular Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China

[2] E-Mails:

[3]Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington, London SW7 2AZ, UK

[4] E-Mails:

[5]Divison of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China

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关键词: Angelica sinensis;    Angelica gigas;    Traditional Chinese Medicine;    NMR;    metabonomics;    metabolomics;   

DOI  :  10.3390/molecules19033460

来源: mdpi

【 摘 要 】

Angelica Radix, the roots of the genus Angelica, has been used for more than 2,000 years as a traditional medicine in Eastern Asia. The Chinese Pharmacopoeia records more than 100 herbal formulae containing Angelica roots. There are two common sources of Angelica roots, Angelica sinensis from China and A. gigas from Korea. The two species of Angelica roots differ in their chemical compositions, pharmacological properties and clinical efficacy. ¹H-NMR metabolic profiling has recently emerged as a promising quality control method for food and herbal chemistry. We explored the use of ¹H-NMR metabolic profiling for the quality control of Angelica Radix. Unlike previous work, we performed the metabolic profiling on hot water extracts, so as to mimic the clinically relevant preparation method. Unsupervised principle component analyses of both the full spectral profile and a selection of targeted molecules revealed a clear differentiation of three types of Angelica roots. In addition, the levels of 13 common metabolites were measured. Statistically significant differences in the levels of glucose, fructose and threonine were found between different sources of Angelica. Ferulic acid, a marker commonly used to evaluate Angelica root, was detected in our samples, but the difference in ferulic acid levels between the samples was not statistically significant. Overall, we successfully applied ¹H-NMR metabolic profiling with water extraction to discriminate all three sources of Angelica roots, and obtained quantitative information of many common metabolites.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.