In Vitro Antiprotozoal Activity of Abietane Diterpenoids Isolated from Plectranthus barbatus Andr.

International Journal of Molecular Sciences

Ramzi A. Mothana¹  Mansour S. Al-Said¹  Nawal M. Al-Musayeib¹  Ali A. El Gamal¹  Shaza M. Al-Massarani¹  Adnan J. Al-Rehaily¹  Majed Abdulkader² 

[1] Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia; E-Mails:;Department of Pharmacognosy, College of Pharmacy, Salman Abdulaziz University, Alkharj 11942, Saudi Arabia; E-Mail:

关键词: Plectranthus barbatus;    antiprotozoal;    abietane-type diterpenoids;    Plasmodium;    Leishmania;    Trypanosoma;    cytotoxicity;   

DOI  :  10.3390/ijms15058360

来源: mdpi

【 摘 要 】

Chromatographic separation of the n-hexane extract of the aerial part of Plectranthus barbatus led to the isolation of five abietane-type diterpenes: dehydroabietane (1); 5,6-didehydro-7-hydroxy-taxodone (2); taxodione (3); 20-deoxocarnosol (4) and 6α,11,12,-trihydroxy-7β,20-epoxy-8,11,13-abietatriene (5). The structures were determined using spectroscopic methods including one- and two-dimensional NMR methods. Compounds (1)–(3) and (5) are isolated here for the first time from the genus Plectranthus. The isolated abietane-type diterpenes tested in vitro for their antiprotozoal activity against erythrocytic schizonts of Plasmodium falciparum, intracellular amastigotes of Leishmania infantum and Trypanosoma cruzi and free trypomastigotes of T. brucei. Cytotoxicity was determined against fibroblast cell line MRC-5. Compound (2) 5,6-didehydro-7-hydroxy-taxodone showed remarkable activity with acceptable selectivity against P. falciparum (IC₅₀ 9.2 μM, SI 10.4) and T. brucei (IC₅₀ 1.9 μM, SI 50.5). Compounds (3)–(5) exhibited non-specific antiprotozoal activity due to high cytotoxicity. Compound (1) dehydroabietane showed no antiprotozoal potential.

【 授权许可】

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