期刊论文详细信息
International Journal of Molecular Sciences
Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding
Shahid Waseem3  Michael A. Allen2  Stefan Schreier1  Rachanee Udomsangpetch3 
[1] Institute for Innovative Learning, Mahidol University, 999 Phuttamonthon 4 Road, Salaya, Nakorn Pathom 73170, Thailand; E-Mail:;Department of Physics, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand; E-Mail:;Department of Pathobiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand; E-Mails:
关键词: high gradient magnetic separation;    bead-cell binding;    iron content;    paramagnetic nanoparticles;    magnetic labeling;    cell separation;   
DOI  :  10.3390/ijms15058821
来源: mdpi
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【 摘 要 】

Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific binding of 150 nm paramagnetic nanobeads to highly purified target and non-target cells. Beads bound to cells were enumerated spectrophotometrically. Results show that the initial bead-cell binding rate and saturation levels depend on initial bead concentration and fit curves of the form A(1 − exp(−kt)). Unspecific binding within conventional experimental time-spans (up to 60 min) was not detectable photometrically. For CD3-positive cells, the probability of specific binding was found to be around 80 times larger than that of unspecific binding.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland

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