International Journal of Molecular Sciences | |
Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding | |
Shahid Waseem3 Michael A. Allen2 Stefan Schreier1 Rachanee Udomsangpetch3 | |
[1] Institute for Innovative Learning, Mahidol University, 999 Phuttamonthon 4 Road, Salaya, Nakorn Pathom 73170, Thailand; E-Mail:;Department of Physics, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand; E-Mail:;Department of Pathobiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand; E-Mails: | |
关键词: high gradient magnetic separation; bead-cell binding; iron content; paramagnetic nanoparticles; magnetic labeling; cell separation; | |
DOI : 10.3390/ijms15058821 | |
来源: mdpi | |
【 摘 要 】
Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific binding of 150 nm paramagnetic nanobeads to highly purified target and non-target cells. Beads bound to cells were enumerated spectrophotometrically. Results show that the initial bead-cell binding rate and saturation levels depend on initial bead concentration and fit curves of the form
【 授权许可】
CC BY
© 2014 by the authors; licensee MDPI, Basel, Switzerland
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