Development and Application of Quantitative Detection Method for Viral Hemorrhagic Septicemia Virus (VHSV) Genogroup IVa

Viruses

Jong-Oh Kim¹  Wi-Sik Kim¹  Si-Woo Kim¹  Hyun-Ja Han²  Jin Woo Kim²  Myoung Ae Park² 

[1] Department of Aqualife Medicine, Chonnam National University, Yeosu 550-749, Korea; E-Mails:;Aquatic Life Disease Control Division, National Fisheries Research and Development Institute, Busan 619-902, Korea; E-Mails:

关键词: viral hemorrhagic septicemia virus (VHSV);    quantitative detection;    real-time PCR;    diagnostic;    olive flounder;   

DOI  :  10.3390/v6052204

来源: mdpi

【 摘 要 】

Viral hemorrhagic septicemia virus (VHSV) is a problematic pathogen in olive flounder (Paralichthys olivaceus) aquaculture farms in Korea. Thus, it is necessary to develop a rapid and accurate diagnostic method to detect this virus. We developed a quantitative RT-PCR (qRT-PCR) method based on the nucleocapsid (N) gene sequence of Korean VHSV isolate (Genogroup IVa). The slope and R² values of the primer set developed in this study were −0.2928 (96% efficiency) and 0.9979, respectively. Its comparison with viral infectivity calculated by traditional quantifying method (TCID₅₀) showed a similar pattern of kinetic changes in vitro and in vivo. The qRT-PCR method reduced detection time compared to that of TCID₅₀, making it a very useful tool for VHSV diagnosis.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.