Phosphorylation Stoichiometries of Human Eukaryotic Initiation Factors

International Journal of Molecular Sciences

Armann Andaya¹  Nancy Villa¹  Weitao Jia¹  Christopher S. Fraser¹ 

[1] Department of Molecular and Cellular Biology, University of California at Davis, Davis, CA 95616, USA;

关键词: mass spectrometry;    eukaryotic initiation factor;    translation;    phosphorylation quantification;   

DOI  :  10.3390/ijms150711523

来源: mdpi

【 摘 要 】

Eukaryotic translation initiation factors are the principal molecular effectors regulating the process converting nucleic acid to functional protein. Commonly referred to as eIFs (eukaryotic initiation factors), this suite of proteins is comprised of at least 25 individual subunits that function in a coordinated, regulated, manner during mRNA translation. Multiple facets of eIF regulation have yet to be elucidated; however, many of the necessary protein factors are phosphorylated. Herein, we have isolated, identified and quantified phosphosites from eIF2, eIF3, and eIF4G generated from log phase grown HeLa cell lysates. Our investigation is the first study to globally quantify eIF phosphosites and illustrates differences in abundance of phosphorylation between the residues of each factor. Thus, identification of those phosphosites that exhibit either high or low levels of phosphorylation under log phase growing conditions may aid researchers to concentrate their investigative efforts to specific phosphosites that potentially harbor important regulatory mechanisms germane to mRNA translation.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.