FEBS Letters | |
Localisation and regulation of the eIF4E‐binding protein 4E‐BP3 | |
Proud, Christopher G1  Kleijn, Miranda1  Wilson, Mary L1  Scheper, Gert C1  Tee, Andrew R1  | |
[1] Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK | |
关键词: eIF4E-binding protein 3; eIF4E-binding protein 1; Primary T cell; Regulation; Nucleus; eIF; eukaryotic initiation factor; FCS; foetal calf serum; m7GTP; 7-methylguanosine triphosphate; 4E-BP; eIF4E-binding protein; HEK293 cells; human embryonic kidney 293 cells; IL-2; interleukin-2; LMB; leptomycin B; Mnk; mitogen-activated protein kinase-interacting kinase; PHA; phytohaemagglutinin; | |
DOI : 10.1016/S0014-5793(02)03694-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The cap-binding protein eIF4E-binding protein 3 (4E-BP3) was identified some years ago, but its properties have not been investigated in detail. In this report, we investigated the regulation and localisation of 4E-BP3. We show that 4E-BP3 is present in the nucleus as well as in the cytoplasm in primary T cells, HEK293 cells and HeLa cells. 4E-BP3 was associated with eIF4E in both cell compartments. Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus.
【 授权许可】
Unknown
【 预 览 】
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RO201912020312524ZK.pdf | 170KB | download |