Localisation and regulation of the eIF4E‐binding protein 4E‐BP3

FEBS Letters

Proud, Christopher G¹  Kleijn, Miranda¹  Wilson, Mary L¹  Scheper, Gert C¹  Tee, Andrew R¹ 

[1] Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK

关键词: eIF4E-binding protein 3;    eIF4E-binding protein 1;    Primary T cell;    Regulation;    Nucleus;    eIF;    eukaryotic initiation factor;    FCS;    foetal calf serum;    m7GTP;    7-methylguanosine triphosphate;    4E-BP;    eIF4E-binding protein;    HEK293 cells;    human embryonic kidney 293 cells;    IL-2;    interleukin-2;    LMB;    leptomycin B;    Mnk;    mitogen-activated protein kinase-interacting kinase;    PHA;    phytohaemagglutinin;   

DOI  :  10.1016/S0014-5793(02)03694-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cap-binding protein eIF4E-binding protein 3 (4E-BP3) was identified some years ago, but its properties have not been investigated in detail. In this report, we investigated the regulation and localisation of 4E-BP3. We show that 4E-BP3 is present in the nucleus as well as in the cytoplasm in primary T cells, HEK293 cells and HeLa cells. 4E-BP3 was associated with eIF4E in both cell compartments. Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus.

【 授权许可】

Unknown