Effects of Pulsed Electromagnetic Field on Differentiation of HUES-17 Human Embryonic Stem Cell Line

International Journal of Molecular Sciences

Yi-Lin Wu²  Shi-Rong Ma¹  Tao Peng⁵  Zeng-Hui Teng³  Xiang-Yan Liang⁴  Guo-Zhen Guo²  Hai-Feng Zhang⁴ 

[1] Department of Pathology and Pathophysiology, the Fourth Military Medical University, Xi’an 710032, China; E-Mail:;Department of Radiation Medicine, the Fourth Military Medical University, Xi’an 710032, China; E-Mails:;Department of Pharmaceutics, the Fourth Military Medical University, Xi’an 710032, China; E-Mail:;Experiment Teaching Center, the Fourth Military Medical University, Xi’an 710032, China; E-Mail:;Department of Neurosurgery, the First Hospital of Jilin University, Changchun 130021, China; E-Mail:

关键词: PEMF;    hESC;    differentiation;   

DOI  :  10.3390/ijms150814180

来源: mdpi

【 摘 要 】

Electromagnetic fields are considered to potentially affect embryonic development, but the mechanism is still unknown. In this study, human embryonic stem cell (hESC) line HUES-17 was applied to explore the mechanism of exposure on embryonic development to pulsed electromagnetic field (PEMF) for 400 pulses at different electric field intensities and the differentiation of HUES-17 cells was observed after PEMF exposure. The expression of alkaline phosphatase (AP), stage-specific embryonic antigen-3 (SSEA-3), SSEA-4 and the mRNA level and protein level of Oct4, Sox2 and Nanog in HUES-17 cells remained unchanged after PEMF exposure at the electric field intensities of 50, 100, 200 or 400 kV/m. Four hundred pulses PEMF exposure at the electric field intensities of 50, 100, 200 or 400 kV/m did not affect the differentiation of HUES-17 cells. The reason why electromagnetic fields affect embryonic development may be due to other mechanisms rather than affecting the differentiation of embryonic stem cells.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.