A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting

International Journal of Molecular Sciences

Francesco Di Girolamo⁴  Andrea Masotti³  Guglielmo Salvatori¹  Margherita Scapaticci²  Maurizio Muraca⁴ 

[1] Neonatal Intensive Care Unit, Department of Medical and Surgical Neonatology, Bambino Gesù Children’s Hospital, IRCCS, Piazza Sant’Onofrio 4, Rome 00165, Italy; E-Mail:;Department of Laboratory Medicine, San Camillo Hospital, Viale Vittorio Veneto 18, Treviso 31100, Italy; E-Mail:;Gene Expression-Microarrays Laboratory, Bambino Gesù Children’s Hospital, IRCCS, Piazza Sant’Onofrio 4, Rome 00165, Italy; E-Mail:;Department of Laboratory Medicine, Bambino Gesù Children’s Hospital, IRCCS, Piazza Sant’Onofrio 4, Rome 00165, Italy; E-Mail:

关键词: milk;    adulteration;    MALDI-TOF MS;   

DOI  :  10.3390/ijms150813697

来源: mdpi

【 摘 要 】

She-donkey’s milk (DM) and goat’s milk (GM) are commonly used in newborn and infant feeding because they are less allergenic than other milk types. It is, therefore, mandatory to avoid adulteration and contamination by other milk allergens, developing fast and efficient analytical methods to assess the authenticity of these precious nutrients. In this experimental work, a sensitive and robust matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling was designed to assess the genuineness of DM and GM milks. This workflow allows the identification of DM and GM adulteration at levels of 0.5%, thus, representing a sensitive tool for milk adulteration analysis, if compared with other laborious and time-consuming analytical procedures.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.