Cordyceps militaris Extract Protects Human Dermal Fibroblasts against Oxidative Stress-Induced Apoptosis and Premature Senescence

Nutrients

Jun Myoung Park¹  Jong Seok Lee¹  Ki Rim Lee²  Suk-Jin Ha² 

[1] Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200701, Korea;

关键词: Cordyceps militaris;    apoptosis;    fibroblast;    oxidative stress;    senescence;   

DOI  :  10.3390/nu6093711

来源: mdpi

【 摘 要 】

Oxidative stress induced by reactive oxygen species (ROS) is the major cause of degenerative disorders including aging and disease. In this study, we investigated whether Cordyceps militaris extract (CME) has in vitro protective effects on hydrogen peroxide-induced oxidative stress in human dermal fibroblasts (HDFs). Our results showed that the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of CME was increased in a dose-dependent manner. We found that hydrogen peroxide treatment in HDFs increased ROS generation and cell death as compared with the control. However, CME improved the survival of HDFs against hydrogen peroxide-induced oxidative stress via inhibition of intracellular ROS production. CME treatment inhibited hydrogen peroxide-induced apoptotic cell death and apoptotic nuclear condensation in HDFs. In addition, CME prevented hydrogen peroxide-induced SA-β-gal-positive cells suggesting CME could inhibit oxidative stress-induced premature senescence. Therefore, these results suggest that CME might have protective effects against oxidative stress-induced premature senescence via scavenging ROS.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.