Vaccines | |
Self-Amplifying Replicon RNA Vaccine Delivery to Dendritic Cells by Synthetic Nanoparticles | |
Kenneth C. McCullough1  Panagiota Milona2  Lisa Thomann-Harwood2  Thomas Démoulins2  Pavlos Englezou2  Rolf Suter1  | |
[1] Institute of Virology and Immunology, CH-3147 Mittelhaeusern, Switzerland; | |
关键词: self-replicating replicon RNA; targeting dendritic cells; nanoparticle delivery; | |
DOI : 10.3390/vaccines2040735 | |
来源: mdpi | |
【 摘 要 】
Dendritic cells (DC) play essential roles determining efficacy of vaccine delivery with respect to immune defence development and regulation. This renders DCs important targets for vaccine delivery, particularly RNA vaccines. While delivery of interfering RNA oligonucleotides to the appropriate intracellular sites for RNA-interference has proven successful, the methodologies are identical for RNA vaccines, which require delivery to RNA translation sites. Delivery of mRNA has benefitted from application of cationic entities; these offer value following endocytosis of RNA, when cationic or amphipathic properties can promote endocytic vesicle membrane perturbation to facilitate cytosolic translocation. The present review presents how such advances are being applied to the delivery of a new form of RNA vaccine, replicons (RepRNA) carrying inserted foreign genes of interest encoding vaccine antigens. Approaches have been developed for delivery to DCs, leading to the translation of the RepRNA and encoded vaccine antigens both
【 授权许可】
CC BY
© 2014 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190020865ZK.pdf | 498KB | download |