Fucoidan, a Sulfated Polysaccharide, Inhibits Osteoclast Differentiation and Function by Modulating RANKL Signaling

International Journal of Molecular Sciences

Young Woo Kim³  Seung-Hoon Baek³  Sang-Han Lee²  Tae-Ho Kim¹  Shin-Yoon Kim³ 

[1] Skeletal Diseases Genome Research Center, Kyungpook National University, Daegu 700-721, Korea;Department of Food Science & Biotechnology, Kyungpook National University, Daegu 702-701, Korea; E-Mail:;Department of Orthopedic Surgery, Kyungpook National University School of Medicine, Daegu 700-422, Korea; E-Mails:

关键词: fucoidan;    osteoclastogenesis;    sulfated polysaccharide;    bone resorption;    RANKL (receptor activator of nuclear factor kappa B (NF-κB) ligand);   

DOI  :  10.3390/ijms151018840

来源: mdpi

【 摘 要 】

Multinucleated osteoclasts differentiate from hematopoietic progenitors of the monocyte/macrophage lineage. Because of its pivotal role in bone resorption, regulation of osteoclast differentiation is a potential therapeutic approach to the treatment of erosive bone disease. In this study, we have found that fucoidan, a sulfated polysaccharide extracted from brown seaweed, inhibited osteoclast differentiation. In particular, addition of fucoidan into the early stage osteoclast cultures significantly inhibited receptor activator of nuclear factor kappa B (NF-κB) ligand (RANKL)-induced osteoclast formation, thus suggesting that fucoidan affects osteoclast progenitors. Furthermore, fucoidan significantly inhibited the activation of RANKL-dependent mitogen-activated protein kinases (MAPKs) such as JNK, ERK, and p38, and also c-Fos and NFATc1, which are crucial transcription factors for osteoclastogenesis. In addition, the activation of NF-κB, which is an upstream transcription factor modulating NFATc1 expression, was alleviated in the fucoidan-treated cells. These results collectively suggest that fucoidan inhibits osteoclastogenesis from bone marrow macrophages by inhibiting RANKL-induced p38, JNK, ERK and NF-κB activation, and by downregulating the expression of genes that partake in both osteoclast differentiation and resorption.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.