X-ray-Induced Changes in the Expression of Inflammation-Related Genes in Human Peripheral Blood

International Journal of Molecular Sciences

Ping Wang²  Fei Guo¹  Lin Han²  Xi𠆚i Wang²  Jie Li²  Yan Guo¹  Yumin Lܲ 

[1] College of Public Health, Zhengzhou University, Zhengzhou 450001, China; E-Mails:;Department of Toxicology, Henan Institute of Occupational Medicine, Zhengzhou 450052, China; E-Mails:

关键词: X-ray;    human peripheral blood;    inflammation-related genes;    quantitative PCR array;   

DOI  :  10.3390/ijms151119516

来源: mdpi

【 摘 要 】

Using quantitative real-time polymerase chain reaction (PCR) array, we explored and compared the expression changes of inflammation-related genes in human peripheral blood irradiated with 0.5, 3, and 10 Gy doses of X-rays 24 h after exposure. Results indicated that the expression of 62 out of 84 genes was significantly altered after X-ray radiation. Among these 62 genes, 35 (such as TNFSF4) are known to be associated with radiation response, but others are novel. At a low radiation dose (0.5 Gy), 9 genes were up-regulated and 19 were down-regulated. With further increased dose to 3 Gy, 8 unique genes were up-regulated and 19 genes were down-regulated. We also identified 48 different genes that were differentially expressed significantly after 10 Gy of irradiation, and among these transcripts, up-regulated genes accounted for only one-third (16 genes) of the total. Of the 62 genes, 31 were significantly altered only at a specific dose, and a total of 10 genes were significantly expressed at all 3 doses. The dose- and time-dependent expression of CCL2 was confirmed by quantitative real-time reverse-transcription PCR. A number of candidate genes reported herein may be useful molecular biomarkers of radiation exposure in human peripheral blood.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.