Target cells capture and detection based on a surface plasmon resonance biosensor

Micro & nano letters

article

X. Chen¹  L.L. Zhang¹  D.F. Cui¹  S. Ge²  J. Zhang²  X. Qiu³ 

[1] State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Institute of Electronics;National Institute of Diagnostics and Vaccine Development in Infectious Diseases, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University Xiamen;Department of Automation, College of Information Science and Technology

关键词: surface plasmon resonance;    cellular biophysics;    biosensors;    target cells capture;    target cells detection;    surface plasmon resonance;    biosensor;    label free mode;    real time mode;    TZM-bl cell line;    lymphocytes;    specific immunity method;    SPRsensing chip;    denaturalised bovine serum albumin layer;    capture capacity;    human peripheral blood;   

DOI  :  10.1049/mnl.2015.0170

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Background & Aims: This prospective, randomized, double-blind study was undertaken to compare the effect of ketorolac or dexmedetomidine to attenuate tourniquet induced rise in blood pressure and heart rate in lower limb orthopedic surgery under general anesthesia. Methodology: One hundred, American Society of Anesthesiologist class I and II patients for elective lower limb surgery under general anesthesia were enrolled, to receive either 30 mg ketorolac (Group KL) or 0.5 µg/kg dexmedetomidine (Group DX) intravenously before inflation of tourniquet. Systolic blood pressure, diastolic blood pressure and heart rate were recorded before induction of anesthesia and at 0, 15, 30, 45, 60 and 75 min after tourniquet inflation, just before tourniquet deflation and at 15 min after tourniquet deflation. Incidence of tourniquet induced hypertension, postoperative analgesic requirements, patient satisfactory score and any side effects were also recorded. Results: Systolic blood pressure, diastolic blood pressure and heart rate significantly increased at 45, 60, 75 min after tourniquet inflation and just before tourniquet deflation in ketorolac group as compared to dexmedetomidine group (P < 0.05). Incidence of tourniquet induced hypertension was more with ketorolac than with dexmedetomidine (P=0.01). The total diclofenac consumption during first 24 hrs of postoperative period was significantly less with ketorolac compared to dexmedetomidine (P=0.001).

【 授权许可】

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