Stem Cells: The Pursuit of Genomic Stability

International Journal of Molecular Sciences

Saranya P. Wyles¹  Emma B. Brandt¹  Timothy J. Nelson¹ 

[1] Center for Regenerative Medicine, Mayo Clinic, Rochester, MN 55901, USA; E-Mails:

关键词: pluripotent stem cells;    multipotent stem cells;    DNA damage response;    reprogramming efficiency;    apoptotic susceptibility;   

DOI  :  10.3390/ijms151120948

来源: mdpi

【 摘 要 】

Stem cells harbor significant potential for regenerative medicine as well as basic and clinical translational research. Prior to harnessing their reparative nature for degenerative diseases, concerns regarding their genetic integrity and mutation acquisition need to be addressed. Here we review pluripotent and multipotent stem cell response to DNA damage including differences in DNA repair kinetics, specific repair pathways (homologous recombination vs. non-homologous end joining), and apoptotic sensitivity. We also describe DNA damage and repair strategies during reprogramming and discuss potential genotoxic agents that can reduce the inherent risk for teratoma formation and mutation accumulation. Ensuring genomic stability in stem cell lines is required to achieve the quality control standards for safe clinical application.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.