Characterisation of Non-Autoinducing Tropodithietic Acid (TDA) Production from Marine Sponge Pseudovibrio Species

Marine Drugs

Catriona Harrington²  F. Jerry Reen²  Marlies J. Mooij²  Fiona A. Stewart²  Jean-Baptiste Chabot²  Antonio F. Guerra⁴  Frank O. Glཬkner⁴  Kristian F. Nielsen³  Lone Gram³  Alan D. W. Dobson¹  Claire Adams²  Fergal O’Gara² 

[1] Environmental Research Institute, University College Cork—National University of Ireland, Cork, Ireland; E-Mail:;BIOMERIT Research Centre, School of Microbiology, University College Cork—National University of Ireland, Cork, Ireland; E-Mails:;Department of Systems Biology, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark; E-Mails:;Microbial Genomics and Bioinformatics Research Group, Max Planck Institute for Marine Microbiology, Bremen D-28359, Germany; E-Mails:

关键词: TDA;    Pseudovibrio;    marine;    antimicrobial;    clinical;   

DOI  :  10.3390/md12125960

来源: mdpi

【 摘 要 】

The search for new antimicrobial compounds has gained added momentum in recent years, paralleled by the exponential rise in resistance to most known classes of current antibiotics. While modifications of existing drugs have brought some limited clinical success, there remains a critical need for new classes of antimicrobial compound to which key clinical pathogens will be naive. This has provided the context and impetus to marine biodiscovery programmes that seek to isolate and characterize new activities from the aquatic ecosystem. One new antibiotic to emerge from these initiatives is the antibacterial compound tropodithietic acid (TDA). The aim of this study was to provide insight into the bioactivity of and the factors governing the production of TDA in marine Pseudovibrio isolates from a collection of marine sponges. The TDA produced by these Pseudovibrio isolates exhibited potent antimicrobial activity against a broad spectrum of clinical pathogens, while TDA tolerance was frequent in non-TDA producing marine isolates. Comparative genomics analysis suggested a high degree of conservation among the tda biosynthetic clusters while expression studies revealed coordinated regulation of TDA synthesis upon transition from log to stationary phase growth, which was not induced by TDA itself or by the presence of the C10-acyl homoserine lactone quorum sensing signal molecule.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.