Ethanol Extract of Haliclona sp. Improved Histological Grade of Mammary Adenocarcinoma in C3H Mice

3rd International Conference on Tropical and Coastal Region Eco Development 2017

地球科学;生态环境科学

Murwani, R.^1,4 ; Trianto, A.^2,4 ; Wijayanti, E.^2 ; Ridlo, A.^2 ; Susilaningsih, N.^3

Faculty of Animal and Agriculture Science, Laboratory of Physiology Dan Biochemistry, Diponegoro University, Jl. Prof. Soedharto, SH, Tembalang, Semarang, Indonesia^1

Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Jl. Prof. Soedharto, SH, Tembalang, Semarang, Indonesia^2

Department of Histology, Faculty of Medicine Diponegoro University, Jl. Prof. Soedharto, SH, Tembalang, Semarang, Indonesia^3

Natural Product Laboratory, Centre for Research and Services, Diponegoro University, Jl. Prof. Soedharto, SH, Tembalang, Semarang, Indonesia^4

关键词: Active substance;    bioactive;    Histological examination;    Initial weights;    mammae;    marine;    Secondary metabolites;    Soft corals;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/116/1/012101/pdf
DOI  :  10.1088/1755-1315/116/1/012101

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The sponge Haliclona sp. contains secondary metabolites belong to alkaloids which are cytotoxic to the human tumor. The following research was conducted to study the effect of a graded level of Haliclona sp. extract on mammary adenocarcinoma in C3H mice. Haliclona sp. was obtained from Bandengan water in Jepara, and the crude extract was prepared by maceration with ethanol. Fifteen C3H mice with an initial weight of 20-25 gram were assigned into control, H-1, and H-2 groups. Control, H1, and H2 groups each received the ethanol extract of 0, 0.15, and 1.5 mg per mouse per day respectively for two weeks. Cancer cells were introduced to all groups from a cancerous donor mouse. The donor cancer cells were injected into each mouse via left or right axilla and allowed to grow. The cancer mass was removed and processed for histological examination, and the cancer growth was determined according to Elston and Ellis criteria. The result showed that histological grade of cancer mass from control group was in grade 2 or differentiated moderately. The histological grade of cancer mass from H-1 and H-2 groups were in grade 1 or similar to a normal cell. Analyses of the data by Kruskal-Wallis showed a significant difference (p