期刊论文详细信息
Marine Drugs
Biosynthesis of Akaeolide and Lorneic Acids and Annotation of Type I Polyketide Synthase Gene Clusters in the Genome of Streptomyces sp. NPS554
Tao Zhou4  Hisayuki Komaki3  Natsuko Ichikawa1  Akira Hosoyama1  Seizo Sato2  Yasuhiro Igarashi4 
[1] Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-4-19 Nishihara, Shibuya-ku, Tokyo 151-0066, Japan; E-Mails:;Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Tokyo Innovation Center, 1-32-3 Nanakuni, Hachioji, Tokyo 192-0991, Japan; E-Mail:;Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan; E-Mail:;Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan; E-Mail:
关键词: polyketide;    marine actinomycete;    Streptomyces;    biosynthesis;    genome;   
DOI  :  10.3390/md13010581
来源: mdpi
PDF
【 摘 要 】

The incorporation pattern of biosynthetic precursors into two structurally unique polyketides, akaeolide and lorneic acid A, was elucidated by feeding experiments with 13C-labeled precursors. In addition, the draft genome sequence of the producer, Streptomyces sp. NPS554, was performed and the biosynthetic gene clusters for these polyketides were identified. The putative gene clusters contain all the polyketide synthase (PKS) domains necessary for assembly of the carbon skeletons. Combined with the 13C-labeling results, gene function prediction enabled us to propose biosynthetic pathways involving unusual carbon-carbon bond formation reactions. Genome analysis also indicated the presence of at least ten orphan type I PKS gene clusters that might be responsible for the production of new polyketides.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.

【 预 览 】
附件列表
Files Size Format View
RO202003190017298ZK.pdf 968KB PDF download
  文献评价指标  
  下载次数:12次 浏览次数:20次