Inhibitory Effect of Duabanga grandiflora on MRSA Biofilm Formation via Prevention of Cell-Surface Attachment and PBP2a Production

Molecules

Carolina Santiago²  Kuan-Hon Lim²  Hwei-San Loh²  Kang Nee Ting¹ 

[1] Faculty of Science, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih, Selangor 43500, Malaysia;

关键词: MRSA;    biofilms;    Duabanga grandiflora;    cell-surface attachment;    PBP2a;   

DOI  :  10.3390/molecules20034473

来源: mdpi

【 摘 要 】

Formation of biofilms is a major factor for nosocomial infections associated with methicillin-resistance Staphylococcus aureus (MRSA). This study was carried out to determine the ability of a fraction, F-10, derived from the plant Duabanga grandiflora to inhibit MRSA biofilm formation. Inhibition of biofilm production and microtiter attachment assays were employed to study the anti-biofilm activity of F-10, while latex agglutination test was performed to study the influence of F-10 on penicillin-binding protein 2a (PBP2a) level in MRSA biofilm. PBP2a is a protein that confers resistance to beta-lactam antibiotics. The results showed that, F-10 at minimum inhibitory concentration (MIC, 0.75 mg/mL) inhibited biofilm production by 66.10%; inhibited cell-surface attachment by more than 95%; and a reduced PBP2a level in the MRSA biofilm was observed. Although ampicilin was more effective in inhibiting biofilm production (MIC of 0.05 mg/mL, 84.49%) compared to F-10, the antibiotic was less effective in preventing cell-surface attachment. A higher level of PBP2a was detected in ampicillin-treated MRSA showing the development of further resistance in these colonies. This study has shown that F-10 possesses anti-biofilm activity, which can be attributed to its ability to reduce cell-surface attachment and attenuate the level of PBP2a that we postulated to play a crucial role in mediating biofilm formation.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.