Sensors | |
Study of the Interaction of Trastuzumab and SKOV3 Epithelial Cancer Cells Using a Quartz Crystal Microbalance Sensor | |
Louise Elmlund1  Camilla K์k2  Teodor Aastrup2  Ian A. Nicholls1  | |
[1]Bioorganic & Biophysical Chemistry Laboratory, Linnæus University Centre for Biomaterials Chemistry, Department of Chemistry & Biomedical Sciences, Linnæus University, SE-39182 Kalmar, Sweden | |
[2] E-Mail: | |
[3]Attana AB, Björnnäsvägen 21, SE-11419 Stockholm, Sweden | |
[4] E-Mails: | |
关键词: quartz crystal microbalance; breast cancer; cell-based biosensor; Herceptin; trastuzumab; HER2; | |
DOI : 10.3390/s150305884 | |
来源: mdpi | |
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【 摘 要 】
Analytical methods founded upon whole cell-based assays are of importance in early stage drug development and in fundamental studies of biomolecular recognition. Here we have studied the binding of the monoclonal antibody trastuzumab to human epidermal growth factor receptor 2 (HER2) on human ovary adenocarcinoma epithelial cancer cells (SKOV3) using quartz crystal microbalance (QCM) technology. An optimized procedure for immobilizing the cells on the chip surface was established with respect to fixation procedure and seeding density. Trastuzumab binding to the cell decorated sensor surface was studied, revealing a mean dissociation constant,
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
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