Radiolabeling of Herceptin with 99mTc as a Her2 tracer

Novelty in Biomedicine

Samira Rasaneh¹  Samira Heydari²  Hossein Rajabi²  Fariba Johari Daha¹ 

[1] Nuclear science and Technology institue;Tarbiat Modares University

关键词: Herceptin;    Trastuzumab;    99mTc;    breast cancer;    Her2;    S-HYNIC;   

DOI  :  

学科分类：生物科学（综合）

来源: Shahid Beheshti University of Medical Sciences

【 摘 要 】

Introduction:Trastuzumab is a monoclonal antibody that is used in treating breast cancer. We labeled this monoclonal antibody with Technetium-99m and performed in vitro and in vivo quality control tests as a first step in the production of a new radiopharmaceutical.Methods:Trastuzumab was labeled with Technetium-99m using Succinimidyl Hydrazinonicotinamide (HYNIC) as chelator. Radiochemical Purity and stability in buffer and serum were determined. Immunoreactivity and toxicity of the complex were tested on SKBR3, MCF7 and A431 breast cancer cell lines. Biodistribution study was performed in normal mice at 4 and 24 h post injection. Results:The radiochemical purity of the complex was 95±1.4%. The stabilities in phosphate buffer and in human blood serum at 24 h post preparation were 85±3.5% and 74±1.2%, respectively. The immunoreactivity of the complex was 86±1.4%. The binding of labeled antibody to the surface of SKBR3, MCF7 and A431 cells were increased by increasing Her2 concentration on the cells surface.Conclusions:The findings showed that the new radiopharmaceutical can be a promising candidate as Her2 antigen scanning for human breast cancer.

【 授权许可】

Unknown