First Evidence that Ecklonia cava-Derived Dieckol Attenuates MCF-7 Human Breast Carcinoma Cell Migration

Marine Drugs

Eun-Kyung Kim¹  Yujiao Tang¹  Yon-Suk Kim⁴  Jin-Woo Hwang⁴  Eun-Ju Choi²  Ji-Hyeok Lee⁵  Seung-Hong Lee¹  You-Jin Jeon⁵  Pyo-Jam Park⁴  Sergey A. Dyshlovoy³ 

[1] Division of Food Bio Science, College of Biomedical and Health Sciences, Konkuk University, Chungju 380-701, Korea; E-Mails:;Division of Sport Science, Konkuk University, Chungju, 380-701, Korea; E-Mail:Division of Food Bio Science, College of Biomedical and Health Sciences, Konkuk University, Chungju 380-701, Korea;;Korea Nokyong Research Center, Konkuk University, Chungju 380-701, Korea; E-Mails:;Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea; E-Mails:

关键词: Ecklonia cava;    phlorotannins;    dieckol;    breast cancer;    migration;   

DOI  :  10.3390/md13041785

来源: mdpi

【 摘 要 】

We investigated the effect of Ecklonia cava (E. cava)-derived dieckol on movement behavior and the expression of migration-related genes in MCF-7 human breast cancer cell. Phlorotannins (e.g., dieckol, 6,6′-biecko, and 2,7″-phloroglucinol-6,6′-bieckol) were purified from E. cava by using centrifugal partition chromatography. Among the phlorotannins, we found that dieckol inhibited breast cancer cell the most and was selected for further study. Radius™-well was used to assess cell migration, and dieckol (1–100 µM) was found to suppress breast cancer cell movement. Metastasis-related gene expressions were evaluated by RT-PCR and Western blot analysis. In addition, dieckol inhibited the expression of migration-related genes such as matrix metalloproteinase (MMP)-9 and vascular endothelial growth factor (VEGF). On the other hand, it stimulated the expression of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2. These results suggest that dieckol exerts anti-breast cancer activity via the regulation of the expressions of metastasis-related genes, and this is the first report on the anti-breast cancer effect of dieckol.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.