Interaction of tRNA with Eukaryotic Ribosome

International Journal of Molecular Sciences

Dmitri Graifer¹  Galina Karpova¹ 

[1] Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, pr. Lavrentieva, 8, 630090 Novosibirsk, Russia; E-Mail:

关键词: tRNA;    eukaryotic ribosome;    eukaryotic translation factors;    ribosomal RNA;    ribosomal proteins;    classical and hybrid tRNA states;   

DOI  :  10.3390/ijms16047173

来源: mdpi

【 摘 要 】

This paper is a review of currently available data concerning interactions of tRNAs with the eukaryotic ribosome at various stages of translation. These data include the results obtained by means of cryo-electron microscopy and X-ray crystallography applied to various model ribosomal complexes, site-directed cross-linking with the use of tRNA derivatives bearing chemically or photochemically reactive groups in the CCA-terminal fragment and chemical probing of 28S rRNA in the region of the peptidyl transferase center. Similarities and differences in the interactions of tRNAs with prokaryotic and eukaryotic ribosomes are discussed with concomitant consideration of the extent of resemblance between molecular mechanisms of translation in eukaryotes and bacteria.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.