Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells

Biosensors

Maria Antonietta Ferrara¹  Giuseppe Di Caprio¹  Stefano Managò²  Annalisa De Angelis²  Luigi Sirleto¹  Giuseppe Coppola¹  Anna Chiara De Luca² 

[1] Institute for Microelectronics and Microsystems, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy; E-Mails:;Institute of Protein Biochemistry, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy; E-Mails:

关键词: spermatozoa;    biophotonics;    sex-identification;    digital holography microscopy;    Raman spectroscopy;    Raman imaging;   

DOI  :  10.3390/bios5020141

来源: mdpi

【 摘 要 】

A full label-free morphological and biochemical characterization is desirable to select spermatozoa during preparation for artificial insemination. In order to study these fundamental parameters, we take advantage of two attractive techniques: digital holography (DH) and Raman spectroscopy (RS). DH presents new opportunities for studying morphological aspect of cells and tissues non-invasively, quantitatively and without the need for staining or tagging, while RS is a very specific technique allowing the biochemical analysis of cellular components with a spatial resolution in the sub-micrometer range. In this paper, morphological and biochemical bovine sperm cell alterations were studied using these techniques. In addition, a complementary DH and RS study was performed to identify X- and Y-chromosome-bearing sperm cells. We demonstrate that the two techniques together are a powerful and highly efficient tool elucidating some important criterions for sperm morphological selection and sex-identification, overcoming many of the limitations associated with existing protocols.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.