Monitoring Human Neutrophil Differentiation by Digital Holographic Microscopy

Frontiers in Physics

Dan Cojoc¹  Maria Augusta do R. B. F. Lima² 

[1] Consiglio Nazionale delle Ricerche, Istituto Officina dei Materiali (CNR-IOM), Trieste, Italy;Department of Physics, University of Trieste, Trieste, Italy;Consiglio Nazionale delle Ricerche, Istituto Officina dei Materiali (CNR-IOM), Trieste, Italy;

关键词: digital holography microscopy;    neutrophil differentiation;    PLB-985 cell line;    cell and nucleus morphology;    label-free;   

DOI  :  10.3389/fphy.2021.653353

来源: Frontiers

【 摘 要 】

We report on the usefulness of digital holographic microscopy (DHM) for the assessment of human neutrophil differentiation from myeloid cells. The cell and nuclear regions have been designated by image segmentation of the optical phase function, and the changes of the cell nucleus morphology in relation to the whole cell morphology have been examined during the process of granulocytic differentiation into mature neutrophils in PLB-985 cell line. Nucleus phase volume and circularity and the ratios between the nucleus and the cell projected area and volume provide a reliable set of parameters to characterize the maturation process. As control, cell differentiation has been monitored in parallel using standard nucleus staining and fluorescence imaging. From this research, it emerged that DHM can be used as a valid label-free solution, alternatively to the standard staining technology.

【 授权许可】

CC BY