SLM Produced Porous Titanium Implant Improvements for Enhanced Vascularization and Osteoblast Seeding

International Journal of Molecular Sciences

Julia Matena⁴  Svea Petersen²  Matthias Gieseke¹  Andreas Kampmann³  Michael Teske²  Martin Beyerbach⁶  Hugo Murua Escobar⁴  Heinz Haferkamp⁵  Nils-Claudius Gellrich³  Ingo Nolte⁴ 

[1] Materials and Processes Department, Laser Zentrum Hannover e.V., D-30419 Hannover, Germany; E-Mail:;Institute for Biomedical Engineering, Rostock University Medical Center, D-18119 Rostock, Germany; E-Mails:;Clinic for Cranio-Maxillo-Facial Surgery, Hannover Medical School, D-30625 Hannover, Germany; E-Mails:;Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, D-30559 Hannover, Germany; E-Mails:;Institut fuer Werkstoffkunde, Leibniz Universitaet Hannover, D-30823 Hannover, Germany; E-Mail:;Institute for Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Foundation, D-30559 Hannover, Germany; E-Mail:

关键词: titanium implant;    selective laser melting;    polycaprolactone;    VEGF;    HMGB1;    CXCL12;    cross section;    live cell imaging;    osteoblast;    cell migration;   

DOI  :  10.3390/ijms16047478

来源: mdpi

【 摘 要 】

To improve well-known titanium implants, pores can be used for increasing bone formation and close bone-implant interface. Selective Laser Melting (SLM) enables the production of any geometry and was used for implant production with 250-µm pore size. The used pore size supports vessel ingrowth, as bone formation is strongly dependent on fast vascularization. Additionally, proangiogenic factors promote implant vascularization. To functionalize the titanium with proangiogenic factors, polycaprolactone (PCL) coating can be used. The following proangiogenic factors were examined: vascular endothelial growth factor (VEGF), high mobility group box 1 (HMGB1) and chemokine (C-X-C motif) ligand 12 (CXCL12). As different surfaces lead to different cell reactions, titanium and PCL coating were compared. The growing into the porous titanium structure of primary osteoblasts was examined by cross sections. Primary osteoblasts seeded on the different surfaces were compared using Live Cell Imaging (LCI). Cross sections showed cells had proliferated, but not migrated after seven days. Although the cell count was lower on titanium PCL implants in LCI, the cell count and cell spreading area development showed promising results for titanium PCL implants. HMGB1 showed the highest migration capacity for stimulating the endothelial cell line. Future perspective would be the incorporation of HMGB1 into PCL polymer for the realization of a slow factor release.

【 授权许可】

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