Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V

Molecules

Sandy Vandermeersch¹  Jorick Vanbeselaere¹  Clément P. Delannoy¹  Aurore Drolez³  Caroline Mysiorek³  Yann Guérardel¹  Philippe Delannoy¹  Sylvain Julien¹  Vito Ferro² 

[1] Structural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d’Ascq, France; E-Mails:Structural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d’Ascq, France;;Blood Brain Barrier Laboratory (LBHE), EA 2465, University of Artois, 62300 Lens, France; E-Mails:

关键词: ST6GalNAc V;    breast cancer;    MDA-MB-231;    α-gangliosides;    GD1α;   

DOI  :  10.3390/molecules20046913

来源: mdpi

【 摘 要 】

α-Series gangliosides define a particular sub-class of glycosphingolipids containing sialic acid α2,6-linked to GalNAc residue that was isolated as a minor compound from the brain. The sialyltransferase ST6GalNAc V was cloned from mouse brain and showed α2,6-sialyltransferase activity almost exclusively for G_M1b, to form G_D1α and is considered as the main enzyme involved in the biosynthesis of α-series gangliosides. Recently, ST6GALNAC5 was identified as one of the genes over-expressed in breast cancer cell populations selected for their ability to produce brain metastasis. However, the capacity of human breast cancer cells to produce α-series gangliosides has never been clearly demonstrated. Here, we show by stable transfection and MS-MS analysis of total glycosphingolipids that ST6GALNAC5 expressing MDA-MB-231 breast cancer cells accumulate G_D1α ganglioside (IV³Neu5Ac₁, III⁶Neu5Ac₁Gg₄-Cer).

【 授权许可】

CC BY   
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