The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2

Marine Drugs

Ji Sook Kang⁴  Il-Whan Choi²  Min Ho Han⁵  Dae-Sung Lee⁵  Gi-Young Kim⁶  Hye Jin Hwang⁴  Byung Woo Kim⁴  Cheol Min Kim³  Young Hyun Yoo¹  Yung Hyun Choi⁴ 

[1] Department of Anatomy and Cell Biology, Dong-A University College of Medicine & Mitochondria Hub Regulation Center, Busan 602-714, Korea;Department of Microbiology, College of Medicine, Inje University, Busan 608-756, Korea; E-Mail:;Department of Biochemistry, Busan National University College of Medicine, Yangsan 626-870, Korea; E-Mail:;Blue-Bio Industry RIC and Anti-AgingResearch Center, Dongeui University, Busan 614-714, Korea; E-Mails:;Marine Biodiversity Institute of Korea, Seocheon 325-902, Korea; E-Mails:;Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Korea; E-Mail:

关键词: Petalonia binghamiae;    oxidative stress;    ROS;    DNA damage;    Nrf2/HO-1;   

DOI  :  10.3390/md13052666

来源: mdpi

【 摘 要 】

This study was designed to examine the protective effects of the marine brown algae Petalonia binghamiae against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms. P. binghamiae methanol extract (PBME) prevented hydrogen peroxide (H₂O₂)-induced growth inhibition and exhibited scavenging activity against intracellular reactive oxygen species (ROS) induced by H₂O₂ in mouse-derived C2C12 myoblasts. PBME also significantly attenuated H₂O₂-induced comet tail formation in a comet assay, histone γH2A.X phosphorylation, and annexin V-positive cells, suggesting that PBME prevented H₂O₂-induced cellular DNA damage and apoptotic cell death. Furthermore, PBME increased the levels of heme oxygenase-1 (HO-1), a potent antioxidant enzyme, associated with the induction of nuclear factor-erythroid 2 related factor 2 (Nrf2). However, zinc protoporphyrin IX, a HO-1 competitive inhibitor, significantly abolished the protective effects of PBME on H₂O₂-induced ROS generation, growth inhibition, and apoptosis. Collectively, these results demonstrate that PBME augments the antioxidant defense capacity through activation of the Nrf2/HO-1 pathway.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.