Inhibition of Oxidative Stress by Low-Molecular-Weight Polysaccharides with Various Functional Groups in Skin Fibroblasts

International Journal of Molecular Sciences

Szu-Kai Chen¹  Chu-Hsi Hsu¹  Min-Lang Tsai¹  Rong-Huei Chen¹ 

[1] Department of Food Science, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 20224, Taiwan; E-Mails:

关键词: antioxidant;    skin fibroblast;    oxidative damage;    oxidative stress;    ROS;    lipid peroxidation;    DNA damage;    agar;    chitosan;    starch;   

DOI  :  10.3390/ijms141019399

来源: mdpi

【 摘 要 】

The aim of this study was to evaluate the in cellulo inhibition of hydrogen-peroxide-induced oxidative stress in skin fibroblasts using different low-molecular-weight polysaccharides (LMPS) prepared from agar (LMAG), chitosan (LMCH) and starch (LMST), which contain various different functional groups (i.e., sulfate, amine, and hydroxyl groups). The following parameters were evaluated: cell viability, intracellular oxidant production, lipid peroxidation, and DNA damage. Trolox was used as a positive control in order to allow comparison of the antioxidant efficacies of the various LMPS. The experimentally determined attenuation of oxidative stress by LMPS in skin fibroblasts was: LMCH > LMAG > LMST. The different protection levels of these LMPS may be due to the physic-chemical properties of the LMPS’ functional groups, including electron transfer ability, metal ion chelating capacities, radical stabilizing capacity, and the hydrophobicity of the constituent sugars. The results suggest that LMCH might constitute a novel and potential dermal therapeutic and sun-protective agent.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland