Immunohistochemical Analysis of Paraoxonases and Chemokines in Arteries of Patients with Peripheral Artery Disease

International Journal of Molecular Sciences

Anna Hernández-Aguilera¹  Julio Sepúlveda²  Esther Rodríguez-Gallego¹  Maria Guirro¹  Anabel Garc໚-Heredia¹  Noemí Cabré¹  Fedra Luciano-Mateo¹  Isabel Fort-Gallifa¹  Vicente Martín-Paredero²  Jorge Joven¹  Jordi Camps¹  Jonathan Golledge³ 

[1] Biomedical Research Unit, Hospital Universitari de Sant Joan, Institut d’Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Catalonia 43201, Spain; E-Mails:;Service of Angiology, Vascular Surgery and Endosurgery, Hospital Universitari Joan XXIII, Institut d’Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Tarragona, Catalonia 43005, Spain; E-Mails:Biomedical Research Unit, Hospital Universitari de Sant Joan, Institut d’Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Catalonia 43201, Spain;

关键词: CCL2;    chemokine receptors;    chemokines;    immunohistochemistry;    paraoxonases;   

DOI  :  10.3390/ijms160511323

来源: mdpi

【 摘 要 】

Oxidative damage to lipids and lipoproteins is implicated in the development of atherosclerotic vascular diseases, including peripheral artery disease (PAD). The paraoxonases (PON) are a group of antioxidant enzymes, termed PON1, PON2, and PON3 that protect lipoproteins and cells from peroxidation and, as such, may be involved in protection against the atherosclerosis process. PON1 inhibits the production of chemokine (C–C motif) ligand 2 (CCL2) in endothelial cells incubated with oxidized lipoproteins. PON1 and CCL2 are ubiquitously distributed in tissues, and this suggests a joint localization and combined systemic effect. The aim of the present study has been to analyze the quantitative immunohistochemical localization of PON1, PON3, CCL2 and CCL2 receptors in a series of patients with severe PAD. Portions of femoral and/or popliteal arteries from 66 patients with PAD were obtained during surgical procedures for infra-inguinal limb revascularization. We used eight normal arteries from donors as controls. PON1 and PON3, CCL2 and the chemokine-binding protein 2, and Duffy antigen/chemokine receptor, were increased in PAD patients. There were no significant changes in C–C chemokine receptor type 2. Our findings suggest that paraoxonases and chemokines play an important role in the development and progression of atherosclerosis in peripheral artery disease.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.