Human Adipose-Derived Mesenchymal Progenitor Cells Engraft into Rabbit Articular Cartilage

International Journal of Molecular Sciences

Wen Wang⁴  Na He⁴  Chenchen Feng⁴  Victor Liu⁴  Luyi Zhang⁴  Fei Wang⁴  Jiaping He⁴  Tengfang Zhu¹  Shuyang Wang¹  Weiwei Qiao²  Suke Li⁴  Guangdong Zhou³  Li Zhang⁴  Chengxiang Dai⁴  Wei Cao⁴ 

[1] Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200433, China; E-Mails:;Animal facility, Shanghai Medical College, Fudan University, Shanghai 200433, China; E-Mail:;Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; E-Mail:;Cellular Biomedicine Group, Palo Alto, CA 94301, USA; E-Mails:

关键词: mesenchymal stem cells;    human adipose-derived mesenchymal progenitor cells;    osteoarthritis;    human leukocyte antigen;   

DOI  :  10.3390/ijms160612076

来源: mdpi

【 摘 要 】

Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.