Anti-Adhesive Activity of Cranberry Phenolic Compounds and Their Microbial-Derived Metabolites against Uropathogenic Escherichia coli in Bladder Epithelial Cell Cultures

International Journal of Molecular Sciences

Dolores González de Llano¹  Adelaida Esteban-Fernández²  Fernando Sánchez-Patán²  Pedro J. Martín-Álvarez²  Mª Victoria Moreno-Arribas²  Bego༚ Bartolomé² 

[1] Institute of Food Science Research (CIAL), CSIC-UAM, C/Nicolás Cabrera 9, 28049 Madrid, Spain;

关键词: UPEC;    uropathogenic Escherichia coli;    UTI;    urinary tract infection;    T-24 cells;    phenolic metabolites;    flavan-3-ols;   

DOI  :  10.3390/ijms160612119

来源: mdpi

【 摘 要 】

Cranberry consumption has shown prophylactic effects against urinary tract infections (UTI), although the mechanisms involved are not completely understood. In this paper, cranberry phenolic compounds and their potential microbial-derived metabolites (such as simple phenols and benzoic, phenylacetic and phenylpropionic acids) were tested for their capacity to inhibit the adherence of uropathogenic Escherichia coli (UPEC) ATCC^®53503™ to T24 epithelial bladder cells. Catechol, benzoic acid, vanillic acid, phenylacetic acid and 3,4-dihydroxyphenylacetic acid showed anti-adhesive activity against UPEC in a concentration-dependent manner from 100–500 µM, whereas procyanidin A2, widely reported as an inhibitor of UPEC adherence on uroepithelium, was only statistically significant (p < 0.05) at 500 µM (51.3% inhibition). The results proved for the first time the anti-adhesive activity of some cranberry-derived phenolic metabolites against UPEC in vitro, suggesting that their presence in the urine could reduce bacterial colonization and progression of UTI.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.