Brighter Fluorescent Derivatives of UTI89 Utilizing a Monomeric vGFP

Pathogens

Majid Eshaghi¹  Kurosh S. Mehershahi¹  Swaine L. Chen¹ 

[1] Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Road, NUHS Tower Block, Level 10, Singapore 119074, Singapore;

关键词: GFP;    uropathogenic Escherichia coli;    urinary tract infection;   

DOI  :  10.3390/pathogens5010003

来源: mdpi

【 摘 要 】

Fluorescent proteins, especially green fluorescent protein (GFP), have been instrumental in understanding urinary tract infection pathogenesis by uropathogenic Escherichia coli (UPEC). We have used a recently developed GFP variant, vsfGFP-9, to create new plasmid- and chromosome-based GFP derivatives of the UPEC strain UTI89. The vsfGFP-9 strains are nearly 10× brighter with no in vitro growth or in vivo virulence defects compared to previously reported GFP-expressing UTI89 strains. The chromosomal vsfGFP-9 strain is equivalent to the wild type UTI89 during in vivo UTI, while both plasmid GFP constructs have an equivalent virulence defect compared to non-plasmid carrying UTI89. These new vsfGFP-9 expressing strains should be useful for further studies of the pathogenesis of UTI89, and similar strategies can be used to create improved fluorescent derivatives of other UPEC strains.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.