The green fluorescent protein chromophore exhibits remarkably different properties upon removal from the protective beta-barrel.This work focuses on the synthesis of these chromophores as wells studying the photophysics as to why they readily deactivate.Following these initial discoveries, these chromophores can be applied to many different environments providing a fluorescence "turn-on" and thus proving to be applicable in a number of different environments and fields.
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Synthesis, photophysics, and application of fluorescent protein chromophore analogs