Nivalenol Has a Greater Impact than Deoxynivalenol on Pig Jejunum Mucosa in Vitro on Explants and in Vivo on Intestinal Loops

Toxins

Sophal Cheat¹  Juliana R. Gerez¹  Juliette Cognié³  Imourana Alassane-Kpembi¹  Ana Paula F. L. Bracarense²  Isabelle Raymond-Letron⁴  Isabelle P. Oswald¹  Martine Kolf-Clauw¹ 

[1]Université de Toulouse, Institut National Polytechnique-Ecole Nationale Vétérinaire (INP-ENVT), Unité Mixte de Recherche UMR 1331 Toxalim, Research Center in Food Toxicology, 23 chemin des Capelles, F-31300 Toulouse, France

[2] E-Mail:

[3]Laboratory of Animal Pathology, Universidade Estadual de Londrina, 86057-990 Londrina, Brazil

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[5]Plate-forme CIRE Chirurgie et Imagerie pour la Recherche et l’Enseignement UMR 085 PRC, INRA, 37380 Nouzilly, France

[6] E-Mail:

[7]INP-ENVT, Université de Toulouse, F-31300 Toulouse, France

关键词: mycotoxins;    jejunum explant;    loops;    deoxynivalenol;    nivalenol;    enterocytes;    histomorphology;   

DOI  :  10.3390/toxins7061945

来源: mdpi

【 摘 要 】

The mycotoxins deoxynivalenol (DON) and nivalenol (NIV), worldwide cereal contaminants, raise concerns for animal and human gut health, following contaminated food or feed ingestion. The impact of DON and NIV on intestinal mucosa was investigated after acute exposure, in vitro and in vivo. The histological changes induced by DON and NIV were analyzed after four-hour exposure on pig jejunum explants and loops, two alternative models. On explants, dose-dependent increases in the histological changes were induced by DON and NIV, with a two-fold increase in lesion severity at 10 µM NIV. On loops, NIV had a greater impact on the mucosa than DON. The overall proliferative cells showed 30% and 13% decrease after NIV and DON exposure, respectively, and NIV increased the proliferative index of crypt enterocytes. NIV also increased apoptosis at the top of villi and reduced by almost half the proliferative/apoptotic cell ratio. Lamina propria cells (mainly immune cells) were more sensitive than enterocytes (epithelial cells) to apoptosis induced by NIV. Our results demonstrate a greater impact of NIV than DON on the intestinal mucosa, both in vitro and in vivo, and highlight the need of a specific hazard characterization for NIV risk assessment.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.