Effect of rhBMP-2 Immobilized Anorganic Bovine Bone Matrix on Bone Regeneration

International Journal of Molecular Sciences

Jung-Bo Huh²  June-Jip Yang³  Kyung-Hee Choi⁴  Ji Hyeon Bae²  Jeong-Yeol Lee³  Sung-Eun Kim¹  Sang-Wan Shin³ 

[1] Department of Orthopedic Surgery & Rare Diseases Institute, Korea University Medical Center, Guro Hospital, Seoul 152-703, Korea;Department of Prosthodontics, Dental Research Institute, Biomedical Research Institute, Institute of Translational Dental Sciences, School of Dentistry, Pusan National University, YangSan 676-870, Korea; E-Mails:;Advanced Prosthodontics, Postgraduate School of Clinical Dentistry, Institute for Clinical Dental Research, Korea University, Seoul 152-703, Korea; E-Mails:;Research Development Institute, Cowellmedi Co., Ltd., Busan 617-801, Korea; E-Mail:

关键词: bone regeneration;    anorganic bovine bone;    Bio-Oss^®;    rhBMP-2;   

DOI  :  10.3390/ijms160716034

来源: mdpi

【 摘 要 】

Anorganic bovine bone matrix (Bio-Oss^®) has been used for a long time for bone graft regeneration, but has poor osteoinductive capability. The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) has been suggested to overcome this limitation of Bio-Oss^®. In the present study, heparin-mediated rhBMP-2 was combined with Bio-Oss^® in animal experiments to investigate bone formation performance; heparin was used to control rhBMP-2 release. Two calvarial defects (8 mm diameter) were formed in a white rabbit model and then implanted or not (controls) with Bio-Oss^® or BMP-2/Bio-Oss^®. The Bio-Oss^® and BMP-2/Bio-Oss^® groups had significantly greater new bone areas (expressed as percentages of augmented areas) than the non-implanted controls at four and eight weeks after surgery, and the BMP-2/Bio-Oss^® group (16.50 ± 2.87 (n = 6)) had significantly greater new bone areas than the Bio-Oss^® group (9.43 ± 3.73 (n = 6)) at four weeks. These findings suggest that rhBMP-2 treated heparinized Bio-Oss^® markedly enhances bone regeneration.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.