Proanthocyanidins Prevent High Glucose-Induced Eye Malformation by Restoring Pax6 Expression in Chick Embryo

Nutrients

Rui-Rong Tan²  Shi-Jie Zhang²  Yi-Fang Li²  Bun Tsoi²  Wen-Shan Huang²  Nan Yao³  Mo Hong²  Yu-Jia Zhai²  Zhong-Fu Mao²  Lu-Ping Tang²  Hiroshi Kurihara²  Qi Wang¹ 

[1] Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Anti-stress and Health Research Center, Pharmacy College, Jinan University, Guangzhou 510632, China; E-Mails:;Guangdong Research Institute of Traditional Chinese Medicine Manufacturing Technology, Guangzhou 510095, China; E-Mail:

关键词: proanthocyanidins;    hyperglycemia;    eye malformation;    oxidative stress;    Pax6;    chick embryo;   

DOI  :  10.3390/nu7085299

来源: mdpi

【 摘 要 】

Gestational diabetes mellitus (GDM) is one of the leading causes of offspring malformations, in which eye malformation is an important disease. It has raised demand for therapy to improve fetal outcomes. In this study, we used chick embryo to establish a GDM model to study the protective effects of proanthocyanidins on eye development. Chick embryos were exposed to high glucose (0.2 mmol/egg) on embryo development day (EDD) 1. Proanthocyanidins (1 and 10 nmol/egg) were injected into the air sac on EDD 0. Results showed that both dosages of proanthocyanidins could prevent the eye malformation and rescue the high glucose-induced oxidative stress significantly, which the similar effects were showed in edaravone. However, proanthocyanidins could not decrease the glucose concentration of embryo eye. Moreover, the key genes regulating eye development, Pax6, was down-regulated by high glucose. Proanthocyanidins could restore the suppressed expression of Pax6. These results indicated proanthocyanidins might be a promising natural agent to prevent high glucose-induced eye malformation by restoring Pax6 expression.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.