Optimization and Evaluation of a Chitosan/Hydroxypropyl Methylcellulose Hydrogel Containing Toluidine Blue O for Antimicrobial Photodynamic Inactivation

International Journal of Molecular Sciences

Chueh-Pin Chen¹  Chien-Ming Hsieh⁵  Tsuimin Tsai²  Jen-Chang Yang⁴  Chin-Tin Chen¹  Michael R. Hamblin³ 

[1] Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan; E-Mail:;Graduate Institute of Biomedical Materials and Tissue Engineering, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan; E-Mail:;Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan; E-Mail;School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan; E-Mail:;Department of Cosmetic Science, Providence University, Taichung City 433, Taiwan; E-Mail:

关键词: chitosan;    hydrogel;    toluidine blue O;    photodynamic inactivation;   

DOI  :  10.3390/ijms160920859

来源: mdpi

【 摘 要 】

Photodynamic inactivation (PDI) combined with chitosan has been shown as a promising antimicrobial approach. The purpose of this study was to develop a chitosan hydrogel containing hydroxypropyl methylcellulose (HPMC), chitosan and toluidine blue O (TBO) to improve the bactericidal efficacy for topical application in clinics. The PDI efficacy of hydrogel was examined in vitro against the biofilms of Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa). Confocal scanning laser microscopy (CSLM) was performed to investigate the penetration level of TBO into viable S. aureus biofilms. Incorporation of HMPC could increase the physicochemical properties of chitosan hydrogel including the hardness, viscosity as well as bioadhesion; however, higher HMPC concentration also resulted in reduced antimicrobial effect. CSLM analysis further demonstrated that higher HPMC concentration constrained TBO diffusion into the biofilm. The incubation of biofilm and hydrogel was further performed at an angle of 90 degrees. After light irradiation, compared to the mixture of TBO and chitosan, the hydrogel treated sample showed increased PDI efficacy indicated that incorporation of HPMC did improve antimicrobial effect. Finally, the bactericidal efficacy could be significantly augmented by prolonged retention of hydrogel in the biofilm as well as in the animal model of rat skin burn wounds after light irradiation.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.