Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications

Journal of Functional Biomaterials

Jana Lisková⁵  Lucie Baლková⁵  Agata L. Skwarczyńska⁶  Olga Musial⁴  Vitaliy Bliznuk¹  Karel De Schamphelaere³  Zofia Modrzejewska²  Timothy E.L. Douglas⁴ 

[1] Department Material Science & Engineering, Ghent University, Technologiepark 903, Zwijnaarde 9052, Belgium; E-Mail:;Department of Environmental Systems Engineering, Faculty of Process and Environmental Engineering, TechnicalUniversity of Łódź, ul. Wólczańska 213, Łódź 90-924, Poland; E-Mail:;Laboratory for Environmental and Aquatic Ecology, Environmental Toxicology Unit (GhEnToxLab), Faculty of Bioscience Engineering, Ghent University, Jozef Plateaustraat 22, Gent 9000, Belgium; E-Mail:;Polymer Chemistry and Biomaterials (PBM) Group, Department of Organic Chemistry, Ghent University, Krijgslaan 281 S4, Gent 9000, Belgium; E-Mail:;Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, Prague, Czech Republic; E-Mail:;Faculty of Civil Environmental Engineering and Architecture, Rzeszow University of Technology, ul. Poznanska 2, Rzeszow 35-084, Poland; E-Mail:

关键词: chitosan;    hydrogel;    cytocompatibility;    magnesium;    mineralization;   

DOI  :  10.3390/jfb6020192

来源: mdpi

【 摘 要 】

Thermosensitive injectable hydrogels based on chitosan neutralized with sodium beta-glycerophosphate (Na-β-GP) have been studied as biomaterials for drug delivery and tissue regeneration. Magnesium (Mg) has been reported to stimulate adhesion and proliferation of bone forming cells. With the aim of improving the suitability of the aforementioned chitosan hydrogels as materials for bone regeneration, Mg was incorporated by partial substitution of Na-β-GP with magnesium glycerophosphate (Mg-GP). Chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were also loaded with the enzyme alkaline phosphatase (ALP) which induces hydrogel mineralization. Hydrogels were characterized physicochemically with respect to mineralizability and gelation kinetics, and biologically with respect to cytocompatibility and cell adhesion. Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability. Cell biological testing showed that both chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were cytocompatible towards MG63 osteoblast-like cells. Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications. However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.