Biocompatibility Evaluation of Dental Luting Cements Using Cytokine Released from Human Oral Fibroblasts and Keratinocytes

Materials

Jae-Sung Kwon¹  Yin-Zhu Piao¹  Sun-A Cho²  Song-Yi Yang¹  Ji Hoon Kim²  Susun An²  Kwang-Mahn Kim¹ 

[1] BK21 PLUS Project, Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Korea;Safety Research Team/Skin Research Division, Amore-Pacific R&D Center, 1920 Yonggu-daero, Giheung-gu, Yongin-si, Gyeonggi-do 446-729, Korea;

关键词: biocompatibility;    cytokine;    cytotoxicity;    dental luting cement;    fibroblast;    inflammation;    keratinocyte;   

DOI  :  10.3390/ma8115372

来源: mdpi

【 摘 要 】

Dental luting cements are commonly used in dentistry for cementation of prosthetic restoration. Many previous studies focused on the measurement of the cell viability as the method of cytotoxicity evaluation during biocompatibility study for the material. In this study, the biocompatibility of various dental luting cements were evaluated using the new method of cytokine release measurement in order to better simulate inflammatory reactions in animal or clinical model using two different oral cells; immortalized human gingival fibroblast and immortalized human oral keratinocytes. Cells were exposed to extractions of various commercially available dental luting cements for different durations. Cytokines of IL-1α and IL-8 were measured from the supernatants of the cells and the results were then compared to the conventional MTT viability test. The result from the conventional cell viability study showed a relatively simple and straight forward indication that only one of the dental luting cements tested in this study was cytotoxic with increasing duration of exposure for both cells. Meanwhile, the result from the cytokine measurement study was much more complex at the time point they were measured, type of cells used for the study and the type of cytokines measured, all of which influenced the interpretation of the results. Hence, the better understanding of the cytokine release would be required for the application in biocompatibility evaluation.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.