Influence of Surfactant and Lipid Type on the Physicochemical Properties and Biocompatibility of Solid Lipid Nanoparticles

International Journal of Environmental Research and Public Health

Carine Dal Pizzol¹  Fabíola Branco Filippin-Monteiro¹  Jelver Alexander Sierra Restrepo³  Frederico Pittella²  Adny Henrique Silva¹  Paula Alves de Souza¹  Angela Machado de Campos¹ 

[1] Departamento de Ciências Farmacêuticas, Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, Brazil; E-Mails:;Departamento de Análises Clínicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG 36036-900, Brazil; E-Mail:;Progama de Pós-Graduação em Engenharia de Materiais, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, Brazil; E-Mail:

关键词: solid lipid nanoparticles;    cytotoxicity;    surfactant;    lipid;    biocompatibility;   

DOI  :  10.3390/ijerph110808581

来源: mdpi

【 摘 要 】

Nine types of solid lipid nanoparticle (SLN) formulations were produced using tripalmitin (TPM), glyceryl monostearate (GM) or stearic acid (SA), stabilized with lecithin S75 and polysorbate 80. Formulations were prepared presenting PI values within 0.25 to 0.30, and the physicochemical properties, stability upon storage and biocompatibility were evaluated. The average particle size ranged from 116 to 306 nm, with a negative surface charge around −11 mV. SLN presented good stability up to 60 days. The SLN manufactured using SA could not be measured by DLS due to the reflective feature of this formulation. However, TEM images revealed that SA nanoparticles presented square/rod shapes with an approximate size of 100 nm. Regarding biocompatibility aspects, SA nanoparticles showed toxicity in fibroblasts, causing cell death, and produced high hemolytic rates, indicating toxicity to red blood cells. This finding might be related to lipid type, as well as, the shape of the nanoparticles. No morphological alterations and hemolytic effects were observed in cells incubated with SLN containing TPM and GM. The SLN containing TPM and GM showed long-term stability, suggesting good shelf-life. The results indicate high toxicity of SLN prepared with SA, and strongly suggest that the components of the formulation should be analyzed in combination rather than separately to avoid misinterpretation of the results.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.