Isoeugenin, a Novel Nitric Oxide Synthase Inhibitor Isolated from the Rhizomes of Imperata cylindrica

Molecules

Hyo-Jin An¹  Agung Nugroho⁴  Byong-Min Song³  Hee-Juhn Park⁵  Derek J. McPhee² 

[1] Department of Pharmacology, College of Korean Medicine, Sangji University, Wonju 220-702, Korea;;Department of Pharmacology, College of Korean Medicine, Sangji University, Wonju 220-702, KoreaDepartment of Forest Science, College of Life Science and Natural Resources, Sangji University, Wonju 220-702, Korea;Department of Agro-Industrial Technology, Faculty of Agriculture, Lambung Mangkurat University, Indonesia 70712, Indonesia;;Department of Pharmaceutical Engineering, College of Health Science, Sangji University, Wonju 220-702, Korea

关键词: Imperata cylindrica;    isoeugenin;    RAW264.7 macrophages;    NO;    HPLC;   

DOI  :  10.3390/molecules201219767

来源: mdpi

【 摘 要 】

Phytochemical studies on the constituents of the rhizomes of Imperata cylindrica (Gramineae) were performed using high-performance liquid chromatography (HPLC). We also aimed to search for any biologically active substance capable of inhibiting nitric oxide (NO) formation in lipopolysaccharide (LPS)-activated macrophage 264.7 cells, by testing four compounds isolated from this plant. Four compounds, including a new chromone, isoeugenin, along with ferulic acid, p-coumaric acid, and caffeic acid were isolated and identified by NMR spectroscopy. The structure of isoeugenin was determined as 7-hydroxy-5-methoxy-2-methylchromone by the 2D-NMR technique. Among the four compounds, isoeugenin has the lowest IC₅₀ value on the inhibition of NO production in LPS-activated macrophage RAW264.7 cells (IC₅₀, 9.33 μg/mL). In addition, isoeugenin significantly suppressed the LPS-induced expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and proinflammatory cytokines mRNA levels. Taken together, these results suggest that the anti-inflammatory activity of isoeugenin is associated with the down-regulation of iNOS, COX-2, and pro-inflammatory cytokines in RAW264.7 cells. Accordingly, our results suggest that the new chromone isoegenin should be considered a potential treatment for inflammatory disease.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.