A Simple and Reliable PDMS and SU-8 Irreversible Bonding Method and Its Application on a Microfluidic-MEA Device for Neuroscience Research

Micromachines

Yufei Ren⁴  Shun-Ho Huang²  Sstien Mosser³  Marc Olivier Heuschkel¹  Arnaud Bertsch⁴  Patrick C. Fraering³  Jia-Jin Jason Chen²  Philippe Renaud⁴ 

[1] Qwane Biosciences SA, Lausanne 1015, Switzerland;Department of Biomedical Engineering, National Cheng Kung University, Tainan 70101, Taiwan;Brain Mind Institute and School of Life Sciences, École Polytechnique Fédérale de Lausanne, EPFL-SV-BMI-CMSN, Station 15, Lausanne 1015, Switzerland;Microsystems Laboratory, École Polytechnique Fédérale de Lausanne, EPFL-STI-IMT-LMIS4, Station 17, Lausanne 1015, Switzerland;

关键词: PDMS;    SU-8;    bonding technology;    surface silanization;    XPS analysis;    tensile strength test;    integrated microfluidic-MEA device;    compartmentalized neural cell culture;    neural activity recording;   

DOI  :  10.3390/mi6121465

来源: mdpi

【 摘 要 】

Polydimethylsiloxane (PDMS) and SU-8 are currently two very commonly used polymeric materials in the microfluidics field for biological applications. However; there is a pressing need to find a simple, reliable, irreversible bonding method between these two materials for their combined use in innovative integrated microsystems. In this paper; we attempt to investigate the aminosilane-mediated irreversible bonding method for PDMS and SU-8 with X-Ray Photoelectron Spectroscopy (XPS) surface analysis and bonding strength tests. Additionally; the selected bonding method was applied in fabricating a microelectrode array (MEA) device, including microfluidic features, which allows electrophysiological observations on compartmentalized neuronal cultures. As there is a growing trend towards microfluidic devices for neuroscience research, this type of integrated microdevice, which can observe functional alterations on compartmentalized neuronal culture, can potentially be used for neurodegenerative disease research and pharmaceutical development.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.