Algal Toxin Azaspiracid-1 Induces Early Neuronal Differentiation and Alters Peripherin Isoform Stoichiometry

Marine Drugs

Linda V. Hjørnevik²  Ann K. Frøyset²  Toril A. Grønset²  Krisna Rungruangsak-Torrissen¹  Kari E. Fladmark² 

[1] Institute of Marine Research, Matre Research Station, N-5984 Matredal, Norway;Department of Molecular Biology, University of Bergen, Thormøhlensgate 55, N-5008 Bergen, Norway;

关键词: azaspiracid;    algal toxin;    neurotoxin;    peripherin;    intermediate filament;    isoform;    PC12 cells;   

DOI  :  10.3390/md13127072

来源: mdpi

【 摘 要 】

Azaspiracid-1 is an algal toxin that accumulates in edible mussels, and ingestion may result in human illness as manifested by vomiting and diarrhoea. When injected into mice, it causes neurotoxicological symptoms and death. Although it is well known that azaspiracid-1 is toxic to most cells and cell lines, little is known about its biological target(s). A rat PC12 cell line, commonly used as a model for the peripheral nervous system, was used to study the neurotoxicological effects of azaspiracid-1. Azaspiracid-1 induced differentiation-related morphological changes followed by a latter cell death. The differentiated phenotype showed peripherin-labelled neurite-like processes simultaneously as a specific isoform of peripherin was down-regulated. The precise mechanism behind this down-regulation remains uncertain. However, this study provides new insights into the neurological effects of azaspiracid-1 and into the biological significance of specific isoforms of peripherin.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.