Journal of Experimental Neuroscience | |
Exploration of Spinal Cord Aging–Related Proteins Using a Proteomics Approach: | |
KoshiroKamiya1  | |
关键词: Aging; proteomics; spinal cord; αB-crystallin; peripherin; | |
DOI : 10.1177/1179069517713019 | |
学科分类:生物科学(综合) | |
来源: Sage Journals | |
【 摘 要 】
How aging affects the spinal cord at a molecular level is unclear. The aim of this study was to explore spinal cord aging–related proteins that may be involved in pathological mechanisms of age-related changes in the spinal cord. Spinal cords of 2-year-old and 8-week-old female Sprague-Dawley rats were dissected from the animals. Protein samples were subjected to 2-dimentional polyacrylamide gel electrophoresis followed by mass spectrometry. Screened proteins were further investigated with immunohistochemistry and Western blotting. Among the screened proteins, we selected α-crystallin B-subunit (αB-crystallin) and peripherin for further investigation because these proteins were previously reported to be related to central nervous system pathologies. Immunohistochemistry and Western blotting revealed significant upregulation of αB-crystallin and peripherin expression in aged rat spinal cord. Further exploration is needed to elucidate the precise mechanism and potential role of these upregulated proteins in spinal cord aging processes.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
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RO201904024746803ZK.pdf | 246KB | download |