Differential expression of αB-crystallin causes maturationdependent susceptibility of oligodendrocytes to oxidative stress

BMB Reports

Eun Young Lee^1¹  Chan Hyung Kim^3²  Hee Yul Ahn^3³  Ji Young Kim^1⁴  Si Wook Kim^2⁵  Hyun Joon Sohn^1⁶ 

[1] Anatomy^2;Department of Anatomy, Chungnam National University School of Medicine, Daejeon 301-747^5;Department of Anatomy, Jeju National University School of Medicine, Jeju 690-756, Korea^6;Departments of^1;Pharmacology, Chungbuk National University School of Medicine, Cheongju 361-763^4;Thoracic and Cardiovascular Surgery^3

关键词: αB-crystallin;   

DOI  :  

学科分类：生物化学/生物物理

来源: Korean Society for Biochemistry and Molecular Biology

【 摘 要 】

Oligodendrocyte precursor cells (OPCs) are most susceptible to oxidative stress in the brain. However, the cause of differences in susceptibility to oxidative stress between OPCs and mature oligodendrocytes (mOLs) remains unclear. Recently, we identified in vivo that αB-crystallin (aBC) is expressed in mOLs but not in OPCs. Therefore, we examined in the present study whether aBC expression could affect cell survival under oxidative stress induced by hydrogen peroxide using primary cultures of OPCs and mOLs from neonatal rat brains. Expression of aBC was greater in mOLs than in OPCs, and the survival rate of mOLs was significantly higher than that of OPCs under oxidative stress. Suppression of aBC by siRNA transfection resulted in a decrease in the survival rate of mOLs under oxidative stress. These data suggest that higher susceptibility of OPCs than mOLs to oxidative stress is due, at least in part, to low levels of aBC expression.

【 授权许可】

Unknown