Exploration of Spinal Cord Aging–Related Proteins Using a Proteomics Approach:

Journal of Experimental Neuroscience

KoshiroKamiya¹ 

关键词: Aging;    proteomics;    spinal cord;    αB-crystallin;    peripherin;   

DOI  :  10.1177/1179069517713019

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

How aging affects the spinal cord at a molecular level is unclear. The aim of this study was to explore spinal cord aging–related proteins that may be involved in pathological mechanisms of age-related changes in the spinal cord. Spinal cords of 2-year-old and 8-week-old female Sprague-Dawley rats were dissected from the animals. Protein samples were subjected to 2-dimentional polyacrylamide gel electrophoresis followed by mass spectrometry. Screened proteins were further investigated with immunohistochemistry and Western blotting. Among the screened proteins, we selected α-crystallin B-subunit (αB-crystallin) and peripherin for further investigation because these proteins were previously reported to be related to central nervous system pathologies. Immunohistochemistry and Western blotting revealed significant upregulation of αB-crystallin and peripherin expression in aged rat spinal cord. Further exploration is needed to elucidate the precise mechanism and potential role of these upregulated proteins in spinal cord aging processes.

【 授权许可】

CC BY