Analysis of Indole Alkaloids from Rhazya stricta Hairy Roots by Ultra-Performance Liquid Chromatography-Mass Spectrometry

Molecules

Amir Akhgari¹  Into Laakso²  Tuulikki Seppänen-Laakso¹  Teijo Yrjönen²  Heikki Vuorela²  Kirsi-Marja Oksman-Caldentey¹  Heiko Rischer¹ 

[1] VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Finland;Division of Pharmaceutical Biosciences, Faculty of Pharmacy, P.O. Box 56, FI-00014 University of Helsinki, Finland;

关键词: Rhazya stricta;    terpenoid indole alkaloids;    ultra-performance liquid chromatography-mass spectrometry;   

DOI  :  10.3390/molecules201219873

来源: mdpi

【 摘 要 】

Rhazya stricta Decne. (Apocynaceae) contains a large number of terpenoid indole alkaloids (TIAs). This study focused on the composition of alkaloids obtained from transformed hairy root cultures of R. stricta employing ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). In the UPLC-MS analyses, a total of 20 TIAs were identified from crude extracts. Eburenine and vincanine were the main alkaloids followed by polar glucoalkaloids, strictosidine lactam and strictosidine. Secodine-type alkaloids, tetrahydrosecodinol, tetrahydro- and dihydrosecodine were detected too. The occurrence of tetrahydrosecodinol was confirmed for the first time for R. stricta. Furthermore, two isomers of yohimbine, serpentine and vallesiachotamine were identified. The study shows that a characteristic pattern of biosynthetically related TIAs can be monitored in Rhazya hairy root crude extract by this chromatographic method.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.