Co-culturing of Catharanthus roseus, Vinca major and Rauwolfia serpentina cell suspensions in shake flask and bioreactor: Production of a novel alkaloid with antioxidant potential

Dynamic Chiropractic

Alka Srivastava¹  Ajay Kumar Mathur¹  Nusrat Masood²  Priyanka Verma³  Singh Arpan⁴ 

[1] PO CIMAP, Lucknow- 226015, India.;Department of Botany, University of Lucknow-226001, India.;Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants, Council of Scientific and Industrial Research (CSIR-CIMAP), Molecular Bio-prospection Department, Central Institute of Medicinal and Aromatic Plants, Council of Scientific and Industrial Research (CSIR-CIMAP), 

关键词: Catharanthus roseus;    Rauwolfia serpentina;    Vinca major;    co-culture;    terpenoid indole alkaloids;    pathway complementation.;   

DOI  :  10.5897/JMPR11.128

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Conditions for co-culturing the cell suspensions ofCatharanthus roseus,Rauwolfia serpentinaandVinca majorin shake flask and bioreactor are described here for the possible complementation of the terpenoid indole alkaloid pathway operating in them. Catharanthus + Rauwolfia or Catharanthus + Vinca cell combinations could be reared on a Murashige and Skoog medium containing 2.0 mg L-1naphthalene acetic acid (NAA) and 0.2 mg L-1kinetin (Kn). A 20- and 40-fold increment in the biomass of these co-cultures was achieved within 30 days in a stirred tank bioreactor. Thin layer chromatography (TLC) of the alkaloid extracts of co-cultures of Rauwolfia + Catharanthus showed the presence of two novel compounds (RF1 and RF2) upon staining with Dragendroff’s reagent. Proton nuclear magnetic resonance(1HNMR), electrospray ionization mass spectrometry (ESI-MS) and ultraviolet (UV) spectral analysis data of these compounds suggested an indole alkaloid identity. Out of these two alkaloids, compound RF1 was found to possess strong antioxidant potential.

【 授权许可】

Unknown