Enzyme Inhibitory Radicinol Derivative from Endophytic fungus Bipolaris sorokiniana LK12, Associated with Rhazya stricta

Molecules

Abdul Latif Khan¹  Liaqat Ali¹  Javid Hussain¹  Tania Shamim Rizvi¹  Ahmed Al-Harrasi¹  In-Jung Lee² 

[1] UoN Chair of Oman’s Medicinal Plants and Marine Natural Products, University of Nizwa, Birkat Al-Mouz, Nizwa 616, Oman; E-Mails:;School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Korea; E-Mail:

关键词: radicinol;    bipolarisenol;    Rhazya stricta;    Bipolaris sorokiniana LK12;    lipid peroxidation;    enzyme inhibition;   

DOI  :  10.3390/molecules200712198

来源: mdpi

【 摘 要 】

Endophytes, living inside plant tissues, play an essential role in plant growth and development, whilst producing unique bioactive secondary metabolites. In the current study, the endophytic fungus Bipolaris sorokiniana LK12 was isolated from the leaves of ethno-medicinal and alkaloidal rich Rhazya stricta. The bulk amount of ethyl acetate extract of fungus was subjected to advance column chromatographic techniques, which resulted in the isolation of a new radicinol derivative, bipolarisenol (1). It was found to be a derivative of radicinol. The structure elucidation was carried out by the combined use of 1D and 2D nuclear magnetic resonance, infrared spectroscopy, mass, and UV spectrometric analyses. The bipolarisenol was assessed for its potential role in enzyme inhibition of urease and acetyl cholinesterase (AChE). Results showed that bipolarisenol significantly inhibited the AChE activity with low IC₅₀ (67.23 ± 5.12 µg·mL⁻¹). Bipolarisenol inhibited urease in a dose-dependent manner with high IC₅₀ (81.62 ± 4.61 µg·mL⁻¹). The new compound also showed a moderate anti-lipid peroxidation potential (IC₅₀ = 168.91 ± 4.23 µg·mL⁻¹). In conclusion, endophytes isolated from medicinal plants possess a unique potential to be considered for future drug discovery.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.