Poly-ε-caprolactone Coated and Functionalized Porous Titanium and Magnesium Implants for Enhancing Angiogenesis in Critically Sized Bone Defects

International Journal of Molecular Sciences

Laura Roland²  Michael Grau²  Julia Matena²  Michael Teske¹  Matthias Gieseke³  Andreas Kampmann⁵  Martin Beyerbach⁴  Hugo Murua Escobar²  Heinz Haferkamp⁶  Nils-Claudius Gellrich⁵  Ingo Nolte² 

[1] Institute for Biomedical Engineering, Rostock University Medical Center, Rostock D-18119, Germany;Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, Hannover D-30559, Germany;Materials and Processes Department, Laser Zentrum Hannover e. V., Hannover D-30419, Germany;Institute for Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Foundation, Hannover D-30559, Germany;Clinic for Cranio-Maxillo-Facial Surgery, Hannover Medical School, Hannover D-30625, Germany;Institut fuer Werkstoffkunde, Leibniz Universitaet Hannover, Garbsen D-30823, Germany;

关键词: titanium implants;    angiogenesis;    poly-ε-caprolactone;    VEGF;    HMGB1;    poly-(3-hydroxybutyrate)/poly-(4-hydroxybutyrate);   

DOI  :  10.3390/ijms17010001

来源: mdpi

【 摘 要 】

For healing of critically sized bone defects, biocompatible and angiogenesis supporting implants are favorable. Murine osteoblasts showed equal proliferation behavior on the polymers poly-ε-caprolactone (PCL) and poly-(3-hydroxybutyrate)/poly-(4-hydroxybutyrate) (P(3HB)/P(4HB)). As vitality was significantly better for PCL, it was chosen as a suitable coating material for further experiments. Titanium implants with 600 µm pore size were evaluated and found to be a good implant material for bone, as primary osteoblasts showed a vitality and proliferation onto the implants comparable to well bottom (WB). Pure porous titanium implants and PCL coated porous titanium implants were compared using Live Cell Imaging (LCI) with Green fluorescent protein (GFP)-osteoblasts. Cell count and cell covered area did not differ between the implants after seven days. To improve ingrowth of blood vessels into porous implants, proangiogenic factors like Vascular Endothelial Growth Factor (VEGF) and High Mobility Group Box 1 (HMGB1) were incorporated into PCL coated, porous titanium and magnesium implants. An angiogenesis assay was performed to establish an in vitro method for evaluating the impact of metallic implants on angiogenesis to reduce and refine animal experiments in future. Incorporated concentrations of proangiogenic factors were probably too low, as they did not lead to any effect. Magnesium implants did not yield evaluable results, as they led to pH increase and subsequent cell death.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.