International Journal of Molecular Sciences | |
Poly-ε-caprolactone Coated and Functionalized Porous Titanium and Magnesium Implants for Enhancing Angiogenesis in Critically Sized Bone Defects | |
Laura Roland2  Michael Grau2  Julia Matena2  Michael Teske1  Matthias Gieseke3  Andreas Kampmann5  Martin Beyerbach4  Hugo Murua Escobar2  Heinz Haferkamp6  Nils-Claudius Gellrich5  Ingo Nolte2  | |
[1] Institute for Biomedical Engineering, Rostock University Medical Center, Rostock D-18119, Germany;Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, Hannover D-30559, Germany;Materials and Processes Department, Laser Zentrum Hannover e. V., Hannover D-30419, Germany;Institute for Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Foundation, Hannover D-30559, Germany;Clinic for Cranio-Maxillo-Facial Surgery, Hannover Medical School, Hannover D-30625, Germany;Institut fuer Werkstoffkunde, Leibniz Universitaet Hannover, Garbsen D-30823, Germany; | |
关键词: titanium implants; angiogenesis; poly-ε-caprolactone; VEGF; HMGB1; poly-(3-hydroxybutyrate)/poly-(4-hydroxybutyrate); | |
DOI : 10.3390/ijms17010001 | |
来源: mdpi | |
【 摘 要 】
For healing of critically sized bone defects, biocompatible and angiogenesis supporting implants are favorable. Murine osteoblasts showed equal proliferation behavior on the polymers poly-ε-caprolactone (PCL) and poly-(3-hydroxybutyrate)/poly-(4-hydroxybutyrate) (P(3HB)/P(4HB)). As vitality was significantly better for PCL, it was chosen as a suitable coating material for further experiments. Titanium implants with 600 µm pore size were evaluated and found to be a good implant material for bone, as primary osteoblasts showed a vitality and proliferation onto the implants comparable to well bottom (WB). Pure porous titanium implants and PCL coated porous titanium implants were compared using Live Cell Imaging (LCI) with Green fluorescent protein (GFP)-osteoblasts. Cell count and cell covered area did not differ between the implants after seven days. To improve ingrowth of blood vessels into porous implants, proangiogenic factors like Vascular Endothelial Growth Factor (VEGF) and High Mobility Group Box 1 (HMGB1) were incorporated into PCL coated, porous titanium and magnesium implants. An angiogenesis assay was performed to establish an
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
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